Publication details
ATP measurements in iron-oxidizing Acidithiobacillus ferrooxidans
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | Advanced Materials Research |
| MU Faculty or unit | |
| Citation | |
| Field | Mining industry incl. coal mining and processing |
| Keywords | Acidithiobacillus ferrooxidans; ATP; iron-oxidizing bacteria |
| Description | We applied a kit for bioluminescent ATP measurement based on the luciferase reaction to Acidithiobacillus ferrooxidans cultures. Determination limit for monitoring cell growth in the culture was 1 . 106 cells per ml. Determination errors varied between 1.5 and 10 % (RSD). ATP formation correlated with cell growth and iron oxidation up to limitation by iron(II). A maximum ATP content was approximately 1 amol per cell under the growth yield on iron of 1 . 1012 cells per mol Fe(II). |
| Related projects: |