Publication details

Study of Interactions between Metallothionein and Cisplatin by using Differential Pulse Voltammetry Brdicka's reaction and Quartz Crystal Microbalance

Authors

HÚSKA Dalibor FABRIK Ivo BALOUN Jiří ADAM Vojtěch MASAŘÍK Michal HUBÁLEK Jaromír VAŠKŮ Anna TRNKOVÁ Libuše HORNA Aleš ZEMAN Ladislav KIZEK René

Year of publication 2009
Type Article in Periodical
Magazine / Source Sensors
MU Faculty or unit

Faculty of Medicine

Citation
Field Biochemistry
Keywords Cancer; Metallothionein; Cisplatin; Protein-Drug Interaction; Voltammetry; Brdicka's reaction; Quartz Crystal Microbalance
Description The interactions between metallothionein (MT) and cisplatin were determined by the adsorptive transfer stripping technique coupled with the differential pulse votlammetry Brdicka's reaction. The signals related to the MT-cisplatin complex appeared at -0.9 V. The formation of this complex depended on the time of interaction between cisplatin and MT. The complex formation was consequently confirmed by quartz crystal microbalance analyses. The formation of this complex was detectable even after a 20 s long interaction. Moreover, we detected presence of MT-cisplatin complex in the blood of male rats treated with this drug.

You are running an old browser version. We recommend updating your browser to its latest version.

More info