Publication details
Seed pod shattering in the genus Lotus and its overcoming
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | Czech Journal of Genetics and Plant Breeding |
| MU Faculty or unit | |
| Citation | |
| Field | Plant cultivation |
| Keywords | interspecific hybridization; Lotus; pod shattering; post-fertilization barriers; pre-fertilization barriers |
| Description | Birdsfoot trefoil (Lotus corniculatus L.) is a perennial species that is known for its outstanding characters as a crucial component of meadows and pasture vegetation and a highly successful fodder crop. However, its cultivation has been limited by the inability to control pod shattering. The anatomic and physiological bases of pod shattering are known and are considered to be controlled by more than one gene. This paper reviews the known causes of pod shattering and potential ways of overcoming pod indehiscence in L. corniculatus. Genetic transformation is possible in the genus Lotus; however, useful genes determining seed pod indehiscent character have not yet been identified. The only way of introducing pod indehiscence characters into L. corniculatus is by interspecific hybridization within the genus Lotus; embryo rescue and protoplast cultures are promising. To determine useful genotypes for crosses, investigations of pre- and post-fertilization barriers are necessary. To that end, we present here a convenient procedure for a whole-mount clearing treatment of immature seeds that leaves the cell walls of tissues intact. This is a useful technique for the study of post-fertilization barriers in Lotus. |
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