Publication details

Characterization of Aeromonas encheleia strains isolated from aquatic environments in the Czech Republic.

Authors

NOVÁKOVÁ Dana ŠVEC Pavel SEDLÁČEK Ivo

Year of publication 2009
Type Article in Periodical
Magazine / Source Letters in Applied Microbiology
MU Faculty or unit

Faculty of Science

Citation
Field Microbiology, virology
Keywords Aeromonas encheleia; aquatic environments; characterization
Description Characterization and identification of Aeromonas strains isolated from surface and underground waters using phenotypic and genotyping methods. Biotyping assigned four strains to A. encheleia, three isolates were identified as A. bestiarum-A. caviae and one strain as A. eucrenophila-A. encheleia. Further characterization grouped the analysed strains together with A. encheleia CCM 4582T and assigned the analysed group as members of A. encheleia using ribotyping, whole-cell protein analysis and ERIC-PCR. The results obtained were verified by DNA gyrase A subunit gene sequencing. All analysed isolates showed unique molecular patterns, except for isolates P 1769 and CCM 7407, which revealed the same EcoRI ribotype profile and proved to be identical strains. Our results imply that A. encheleia occur in unpolluted surface as well as in underground waters. This is the first report of the isolation and identification of A. encheleia in the Czech Republic.
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