Publication details
miRNA expression profile of regulatory T cells in patiens with diabetes mellitus type I
| Authors | |
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| Year of publication | 2009 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | MicroRNA (miRNA) are small non-coding RNA molecules, that have a potential to regulate the expression of many genes. Type I diabetes (T1D) is an autoimmune disease resulting from the destruction of pancreatic beta cells by cytotoxic T cells. Regulatory T cells (Treg) are specialised lymphocytes preventing from development of autoimmune disorders. Study on transgenic mouse with conditional Dicer knockout Treg revealed that this Treg cell-specific miRNA ablation results in the loss of suppressor function and development of fatal systemic autoimmune disease in vivo. Therefore we can assume, that the functional defect of Treg in T1D patients can be caused by different expression miRNA profile in these cells. The aim of our study was to establish expression profile of miRNA in patients with T1D and compare it with healthy donors. Six diabetic patients and 6 healthy volunteers provided peripheral blood for flow cytometry analyses and separation of Treg (CD3+, CD4+, CD25+, CD127-) and non-Treg cells (CD3+, CD4+, CD25-). Total RNA rich in small RNAs was isolated and used for TaqMan Human MicroRNA Array that analysed expression of 384 miRNA in Treg and non-Treg cells. The data were subsequently processed by HCL, SAM analysis. Different miRNA expression patterns were identified in Treg and non-Treg populations as well as in Treg cells between T1D and healthy individuals. We aimed to identify miRNA specific in pathogenesis of diabetes mellitus which could be used in the future as therapeutic targets of diabetic patients. |