Publication details
microRNA-342, microRNA-191 and microRNA-510 are differentially expressed in T regulatory cells of type 1 diabetic patients
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Cellular Immunology |
| MU Faculty or unit | |
| Citation | |
| Web | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WCF-4XJ17PW-4&_user=835458&_coverDate=12%2F31%2F2010&_rdoc=4&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236737%232010%23997399997%231577274%23FLA%23display%23Volume)&_cdi=6737&_sort=d&_docancho |
| Field | Other specializations of internal medicine |
| Keywords | microRNA; Expression profile; Regulatory T cells; Diabetes mellitus type 1 |
| Description | We performed miRNAs profiling in a population of regulatory T celss (Tregs) separated from peripheral blood of five type 1 diabetic patients and six healthy donors. For more detailed molecular char- acterisation of Tregs, we additionally compared miRNAs expression profiles of Tregs and conventional T cells. Tregs were isolated according to CD3+, CD4+, CD25hi+ and CD127low by flow cytometry, and miRNA expression profiling was performed using TaqMan Array Human MicroRNA Panel-1 (384-well low density array). In Tregs of diabetic patients we found significantly increased expression of miRNA-510 (p = 0.05) and decreased expression of both miRNA-342 (p< 0.0001) and miRNA-191 (p = 0.0079). When comparing Tregs and T cells, we revealed that Tregs had significant higher expression of miRNA-146a and lower expression of eight specific miRNAs (20b, 31, 99a, 100, 125b, 151, 335, and 365). This is the first study demonstrating changes in miRNA expression profiles occurring in Tregs of T1D patients and a miRNAs signature of adult Tregs. |