Publication details
Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Cell Transplantation |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | human embryonic stem cells; neural differentiation; fluorescent activated cell sorting; RT-PCR; transplantation |
| Description | Here, we demonstrate the derivation of highly enriched and expandable populations of proliferating neural precursors from the CCTL14 line of human embryonic stem cells (hESCs). These neural precursors could differentiate in vitro into functionally active neurons, as confirmed by immunohistochemical staining and electrophysiological analysis. Neural cells differentiated in vitro from hESCs exhibit broad cellular heterogeneity with respect to developmental stage and lineage specification. The analyzed cells were used for transplantation into the injured rodent brain; the tumorigenicity of the transplanted cells was apparently eliminated following long-term culture. These results complete the characterization of the CCTL14 line of hESCs and provide a framework for developing cell selection strategies for neural cell-based therapies.. |