Publication details
Jak zabránit zavíječi voskovému (G. mellonella) ve spřádání kokonu?
| Title in English | How to block spinning of the cocoon in the greater wax moth (G. mellonella)? |
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| Authors | |
| Year of publication | 2010 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Larvae of the greater wax moth (Galleria mellonella L., Lepidoptera, Pyralidae) spin a cocoon before pupation. Salivary glands of larvae produce liquid which changes after hardening to fibrinous fibres. These fibres are then sticked with sericins and deposited in several layers into cocoon. The greater wax moth is most widely used representative of wax moths family, but in many experiments using its pupae (like studies of metamorphosis, pupae infections etc.), the pupae without cocoon would be much better to work with. It is known that function of salivary glands can be influenced by heat shock; simple souse of larvae to water of defined temperature results in loss of ability to spin cocoon. We tested temperature of water within the range of 52-56 C and time range 5-30 s. As the best water temperature 56 C and duration of submersion 15 s was selected; after this treatment 75 % of larvae were unable to spin cocoon and pupated without it. Longer time increases mortality of larvae and shorter time interval or lower temperature weakens the demanded effect. Even after treatment the cocoon is still present in 25 % or more larvae, but only in its reduced form. Eclosion of adults from pupae without cocoons acquired after heat shock was not affected. This work was supported by grant GACR 206/09/P470. |
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