Ribotyping and biotyping of Lactobacillus helveticus from the koumiss.

• Authors: SEDLÁČEK Ivo; NOVÁKOVÁ Dana; ŠVEC Pavel
• Year of publication: 2010
• Type: Article in Periodical
• Magazine / Source: European Food Research Technology
• MU Faculty or unit: Faculty of Science
• Field: Microbiology, virology
• Keywords: Lactobacillus helveticus-Ribotyping-Rep-PCR-Koumiss-Biotyping-Identification
• Description: Lactobacillus helveticus, a homofermentative thermophilic lactobacillus commonly occurring in cheeses, has been isolated as the prevailing species in natural koumiss in this work. The species identification of six studied L. helveticus strains was based on rep-PCR fingerprinting with (GTG)5 primer. Biotyping (API 50CH kit, conventional tests) shown phenotypic heterogeneity among isolates and enabled the identification to the genus Lactobacillus only. Ribotyping with restriction enzyme EcoRI yielded a strain - specific restriction patterns and allowed good strain differentiation. Despite the low number of strains analyzed, the ribotype data showed certain heterogeneity, that seemed to be not only strain dependent, but also related to the source of isolates. We concluded that analyzed isolates from koumiss represent a new ecovar, L. helveticus ecovar Koumiss.

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