Publication details
Capillary Electromigration Separation of Proteins and Microorganisms Dynamically Modified by Chromophoric Nonionogenic Surfactant
| Authors | |
|---|---|
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | Analytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | Capillary Electromigration Techniques; Proteins; Microorganisms; Dynamic Modification; Chromophoric Non-ionogenic Surfactant |
| Description | Chromophoric non-ionogenic surfactant poly(ethylene glycol) 3-(2-hydroxy-5-n-octylphenylazo)-benzoate, HOPAB, has been prepared and used as a buffer additive for a dynamic modification of proteins and/or microorganisms including Escherichia coli, Staphylococcus epidermidis (biofilm positive and negative), the strains of yeast cells, Candida albicans, Candida parapsilosis (biofilm positive and negative) during a capillary electrophoresis and a capillary isoelectric focusing with UV detection at 326 nm. Minimum detectable amount has been assessed lower than picograms of proteins and lower than a hundred cells injected into a separation capillary. The introduced labeling method facilitates CIEF separation of microorganisms from the clinical sample of the infected urine at their clinically important level in the pH gradient pH range 2-5 and their subsequent cultivation. |
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