Mitochondrial Toxicity of Microcystin-LR on Cultured Cells: Application to the Analysis of Contaminated Water Samples

• Authors: JASIONEK G.; ZHDANOV A.; DAVENPORT J.; BLAHA L.; PAPKOVSKY D.B.
• Year of publication: 2010
• Type: Article in Periodical
• Magazine / Source: Environmental Science & Technology
• MU Faculty or unit: Faculty of Science
• Field: Environment influence on health
• Keywords: microcystin; cyanobacteria; bioanalysis; mitochondria
• Description: We analyzed the effects of MC-LR on primary hepatocytes, HepG2, and Jurkat T cells, and isolated rat liver mitochondria by measuring changes in 02 consumption by optical oxygen sensing technique. The respiratory response did not coincide with changes in viability, total cellular ATP, extracellular acidification, ROS formation, or protein phosphorylation, which were detectable at higher MC-LR doses. Such prominent effect on cellular respiration was therefore used for the detection of MC-LR in environmental samples.

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