Publication details

Mitochondrial Toxicity of Microcystin-LR on Cultured Cells: Application to the Analysis of Contaminated Water Samples

Authors

JASIONEK G. ZHDANOV A. DAVENPORT J. BLAHA L. PAPKOVSKY D.B.

Year of publication 2010
Type Article in Periodical
Magazine / Source Environmental Science & Technology
MU Faculty or unit

Faculty of Science

Citation
Field Environment influence on health
Keywords microcystin; cyanobacteria; bioanalysis; mitochondria
Description We analyzed the effects of MC-LR on primary hepatocytes, HepG2, and Jurkat T cells, and isolated rat liver mitochondria by measuring changes in 02 consumption by optical oxygen sensing technique. The respiratory response did not coincide with changes in viability, total cellular ATP, extracellular acidification, ROS formation, or protein phosphorylation, which were detectable at higher MC-LR doses. Such prominent effect on cellular respiration was therefore used for the detection of MC-LR in environmental samples.
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