The use of Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry whole cell protein/peptide profiles for identification and characterization of lactic acid bacteria

• Authors: TESHIM Andrea; ŠEDO Ondrej; SEDLÁČEK Ivo; ZDRÁHAL Zbyněk; DRÁB Vladimír
• Year of publication: 2010
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: In dairy industry there is a great need for proper identification and characterization of relevant milk cultures. Matrix Assisted Laser Desorption/Ionization - Time of Flight Mass Spectrometry (MALDI MS) provides unique and highly reproducible fingerprints of bacteria (protein/peptide profiles) characteristic for particular species or strains. Identification is based on comparison of bacterial sample profiles with a database of the profiles of type and reference cultures and can be applied for classification of novel strains. The aim of our study was to assess capabilities of MALDI MS profiling for reliable characterization and identification of lactic acid bacteria. Cluster analysis showed the capability of MALDI-MS to differentiate and to classify strains at the species and in case of particular Lactobacillus groups at the sub-species level. The main advantage of MALDI MS approach is its rapidity and the possibility of cultivable bacteria identification on the genus, species and subspecies level. This technique could be applied for identification and characterization of novel probiotic strains.

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