Determination of Metallothionein in prostate tumor cell lines

• Authors: GUMULEC Jaromír; MASAŘÍK Michal; KUCHTÍČKOVÁ Šárka; KUDLÁČKOVÁ Veronika; JURAJDA Michal; PAVLÍK Dušan; ROVNÝ Arne; HRABEC Roman; KŘÍŽKOVÁ Soňa; KIZEK René
• Year of publication: 2010
• Type: Conference abstract
• MU Faculty or unit: Faculty of Medicine
• Citation: GUMULEC, Jaromír, Michal MASAŘÍK, Šárka KUCHTÍČKOVÁ, Veronika KUDLÁČKOVÁ, Michal JURAJDA, Dušan PAVLÍK, Arne ROVNÝ, Roman HRABEC, Soňa KŘÍŽKOVÁ and René KIZEK. Determination of Metallothionein in prostate tumor cell lines. In XIV. Setkání biochemiků a molekulárních biologů. 2010. ISBN 978-80-210-5164-5.
• Description: Prostate carcinoma (PCa) is the most common tumor and second leading cause of death due to cancer of men. Proteins such as metallothionein (MT) or alpha-methyl-CoA-racemase (AMACR) are highly overexpressed in prostate carcinoma. Althrough the role of these proteins in tumour tissue remains still unclear, it fulfills the requirements of higher specificity, which AMACR achieves at almost 100%. We have analyzed both proteins from prostate cancer cell lines LNCaP, PC-3 and 22RVL, compared to healthy prostate cell line PNT1A and then from PCa patients' serum compared to healthy serum controls. We've used immunohistochemical analysis, immunoprecipitation with magnetic nanoparticles, PAGE and dot blots. In all of these methods, we've found significant increase of expression in these samples.

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