Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

• Authors: PROKOP Zbyněk; SATO Yukari; BREZOVSKÝ Jan; MOZGA Tomáš; CHALOUPKOVÁ Radka; KOUDELÁKOVÁ Táňa; JEŘÁBEK Petr; ŠTĚPÁNKOVÁ Veronika; NATSUME Rio; LEEWEN J.G.E.; JANSSEN D,B.; FLORIAN J.; NAGATA Yuji; SENDA T.; DAMBORSKÝ Jiří
• Year of publication: 2010
• Type: Article in Periodical
• Magazine / Source: Angewandte Chemie International Edition
• MU Faculty or unit: Faculty of Science
• web: http://loschmidt.chemi.muni.cz/peg/pdf/angewandte2010.pdf
• Field: Biochemistry
• Keywords: Enantioselectivity; Haloalkane Dehalogenases; protein engineering
• Description: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.

Related projects

• Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)
• Center of Biocatalysis and Biotransformation
• Evolution of substrate specificity in enzymes acting on xenobiotic compounds