Biomarkers of metastatic potential in low grade breast cancer

• Authors: BOUCHAL Pavel; MUDROCHOVÁ Monika; STRUHÁROVÁ Iva; ROUMELIOTIS Theodoros; HERNYCHOVÁ Lenka; HRSTKA Roman; GARBIS Spiros D.; NENUTIL Rudolf; VOJTĚŠEK Bořivoj
• Year of publication: 2010
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: Our effort is focused on discovery, evaluation and validation of perspective protein markers of metastatic potential in low grade breast cancer biopsies. Three proteomics approaches have been involved in complex protein composition analysis of clinically and pathologically well characterized set of tissues: SELDI-TOF MS, 2-D PAGE and iTRAQ-2DLC-MS/MS. This combined approach enabled us to simultaneously identify and quantify more than 600 proteins using LC-MS/MS and to detect more than 800 protein spots using 2-D PAGE followed by MS/MS protein identification. The results involving differential levels of several biologically relevant groups of proteins are discussed on the poster.

Related projects

• Proteins in metabolism and interaction of organisms with the environment
• Studium molekulárních mechanismů časného metastazování do lymfatických uzlin u karcinomu prsu nízkého stupně malignity pomocí proteomických technik