Publication details
Comparison of gene expression profiling between amplified clinical samples using cDNA microarrays
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | Advances in Molecular and Cancer Biology, 2010 |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | microarrays - cDNA - amplification - pulmonary cells |
| Description | Comparison of gene expression profiles obtained using microarrays technology applied to clinical samples ranks among current issues especially if amplification is needed for obtaining a sufficient amount of input RNA. We have designed a protocol for (i) convenient isolation of pure RNA from a small amount of cells, (ii) precise RNA amplification and (iii) determination of obtained microarray data. Pulmonary cells represented the clinical samples of given characteristic in our study. |
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