Publication details
Direct Voltammetric Analysis of DNA Modified with Enzymatically Incorporated 7-Deazapurines
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Periodical |
| Magazine / Source | Analytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| Field | Biophysics |
| Keywords | 7-deozapurines; voltammetric analysis; carbon electrode |
| Description | Nucleic acids studies use 7-deazaguanine and 7-deazaadenine as analogues of natural purine bases. We showed that enzymatic incorporation of 7-deazaguanine and 7-deazaadenine can easily be monitored by simple ex situ voltammetric analysis of the modified DNA at carbon electrodes. 7-deazaguanine was shown as an attractive electroactive marker for DNA, efficiently incorporable by PCR. |