Publication details

The Expression of NADPH Oxidases and Production of Reactive Oxygen Species by Human Lung Adenocarcinoma Epithelial Cell Line A549

Authors

KOLÁŘOVÁ Hana BINÓ Lucia PEJCHALOVÁ Kateřina KUBALA Lukáš

Year of publication 2010
Type Article in Periodical
Magazine / Source Folia biologica
MU Faculty or unit

Faculty of Science

Citation
Web http://fb.cuni.cz/Data/files/folia_biologica/volume_56_2010_5/FB2010A0029.pdf
Field Physiology
Keywords HYDROGEN-PEROXIDE; HOST-DEFENSE; NITRIC-OXIDE; SUPEROXIDE; PHAGOCYTES; CHEMILUMINESCENCE; DIFFERENTIATION; L-012
Description Human lung epithelial cell line A549 was found to significantly express NOX1, NOX2, DUOX1 and DUOX2 and ROS production was detected with fluorometric probes 2’,7’-dichlorofluorescein- diacetate, dihydroethidium, and amplex red or colorimetric probe nitrobluetetrazolium. These results revealed differences among methods used for ROS formation measurement by cell line A549 and highlighted the sensitivity of fluorometric determination for this purpose.

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