Publication details

Occurrence and characteristics of Listeria monocytogenes in ready-to-eat food from retail market in the Czech Republic

Authors

GELBÍČOVÁ Tereza KARPÍŠKOVÁ Renata

Year of publication 2009
Type Article in Periodical
Magazine / Source Czech Journal of Food Sciences
MU Faculty or unit

Faculty of Science

Citation
Keywords L. monocytogenes, foodstuff, typing methods, hygiene
Description The study objectives were to test ready-to-eat food from the retail market in the Czech Republic for the presence of L. monocytogenes and, based on typing methods, to investigate probable causes of contamination. A total 2180 samples of ready-to-eat food (meat, dairy, fish, delicatessen and confectionery products and fresh fruit and vegetables) were analyzed qualitatively and quantitatively. L. monocytogenes isolates were characterized by serotyping and macrorestriction analysis after digestion with the restriction enzyme AscI. In 2004–2008 L. monocytogenes was most often detected in delicatessen (5.2%), meat (3.4%) and dairy products (1.8%). In the analyzed samples, L. monocytogenes was mostly present at counts lower than 102 CFU.g-1. Only in 2004, higher counts of L. monocytogenes were found in two heat-processed meat products (103 CFU.g-1). The obtained macrorestriction patterns helped in tracing the source of contamination and routes of the spread of L. monocytogenes in the manufacturing plant and retail market.

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