Publication details

Prevalence Listeria monocytogenes ve zrajících sýrech z tržní sítě České republiky

Title in English Prevalence of Listeria monocytogenes in rippening cheese from retail market in the Czech Republic
Authors

GELBÍČOVÁ Tereza KARPÍŠKOVÁ Renata

Year of publication 2008
Type Article in Proceedings
Conference X. Konference mladých vědeckých pracovníků s mezinárodní účastí
MU Faculty or unit

Faculty of Science

Citation
Keywords serotyping, PCR, macro-restriction analysis, pulsotypes
Description The aim of this study was to detect and characterise L. monocytogenes in rippening cheese from retail market in the Czech Republic. A total of 142 samples originating from the Czech and foreign producers were tested in the year 2007. The detection and enumeration of L. monocytogenes were performed according to ČSN EN/ISO 11290 - 1, 2. L. monocytogenes isolates were characterised by serotyping using the slide agglutination method and multiplex PCR assay and typing has been performed by restriction analysis using pulsed-field gel electrophoresis (PFGE). L. monocytogenes was detected in 12 samples of czech origin: in 17% (4/24) of smeared cheese samples, 15% (7/48) of blue-veined cheese and 1% (1/70) of cheese with the mould on the surface. All isolates belonged to serotype 1/2a. Using macro-restriction analysis, 3 different pulsotypes have been detected. Although positive L. monocytogenes samples were detected on retail level, none of them exceeded the count of 100 CFU.g-1. Specific pulsotypes have been detected for each of the individual cheese producers.

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