Publication details
High resolution AFM as a tool for analysis of short-chain DNA structure and properties
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Proceedings |
| Conference | Biosurf 2007, Zurich |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | AFM microscope DNA mica HOPG |
| Description | Immobilization of biomolecules on surfaces while keeping the maximum conformation flexibility of the molecules is one of the most important techniques for atomic force microscopy imaging. When the DNA molecules are studied withAtomic Force Microscope (AFM) either HOPG (Highly Ordered Pyrolytic Graphite) or freshly cleaved mica is used as asubstrate because they show atomically flat surface. DNA molecules can be adsorbed to the graphite surface, howeverthis kind of supporting material is very expensive. Mica shows high flatness, unfortunately it can not be used for for DNAimmobilization without specific treatment, as both mica and DNA are negatively charged in standard buffer solutions.Scanning probe microscopy (SPM) are based on the ability to detect a local property of a surface by means of spatiallycontrolled sensor or probe. Typically, the sensor is scanned over the area of interest so it is only transiently localized inthe neighbourhood of a given point on the surface. |
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