Flow Cytometric Phenotyping and Analysis of T Regulatory Cells in Multiple Myeloma Patients

• Authors: MUTHU RAJA Karthick Raja; KOVÁŘOVÁ Lucie; ŠTOSSOVÁ Jana; HÁJEK Roman
• Year of publication: 2011
• Type: Article in Periodical
• Magazine / Source: Klinická onkologie
• MU Faculty or unit: Faculty of Medicine
• Field: Oncology and hematology
• Keywords: Tregs; flow cytometry; multiple myeloma; thalidomide
• Description: Multiple myeloma (MM) is a plasma cell (PC) disorder and associated with immune impairments. Flow cytometry based phenotyping and quantification of regulatory T cells (Tregs) enable to monitor the immune status of myeloma patients. Apart from enumeration of Tregs, functional characterization using proliferation or suppression assay adds key value in demonstrating the functional value of Tregs. Our study revealed that in MM patients Tregs are elevated compared to healthy subjects, which demonstrate the immune deregulation in MM.

Related projects

• University Research Centre - Czech Myeloma Group
• From classic prognostic markers to clinical applications in selected pharmacogenomic and pharmacoproteomic projects in multiple myeloma and monoclonal gammapathies
• Vytvoření prognostického panelu u pacientů s monoklonální gamapatií nejasného významu s cílem zabránění transformace v maligní onemocnění
• Analýza klonálních progenitorů plazmatických buněk u monoklonálních gamapatií