Nový lektin ze světélkující bakterie Photorhabdus luminescens

• Title in English: New lectin from bioluminescent bacterium Photorhabdus luminescens
• Authors: ROZUMOVÁ Petra; WIMMEROVÁ Michaela
• Year of publication: 2011
• Type: Conference abstract
• MU Faculty or unit: Faculty of Science
• Description: The protein PLU from P. luminescens has a strong sequence homology with characterized lectin BC2L-C-nt from the human opportunistic pathogen Burkholderia cenocepacia. So far, PLU is the only one supposed homologous of BC2L-C-nt lectin. Function studies were performed using surface plasmon resonance and isothermal titration calorimetry, which allowed identifying binding properties and thermodynamic parameters of interaction with saccharides. Preliminary information about a quaternary structure of the protein was obtained using dynamic light scattering, crosslinking with bifunctional reagents and analytical ultracentrifugation.

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