Publication details
Využití metody PCR-RFLP k detekci potenciálně invazivních kmenů L. monocytogenes
| Title in English | Use of PCR-RFLP for the detection of potentially invasive strains of L. monocytogenes |
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| Authors | |
| Year of publication | 2011 |
| Type | Article in Periodical |
| Magazine / Source | Chemické listy |
| MU Faculty or unit | |
| Citation | |
| Field | Microbiology, virology |
| Keywords | pathogenicity; internalin A; polymerase chain reaction; restriction cleavage |
| Description | Internalin A, encoded by the gene inlA, is the key virulence factor, which allows crossing the intestinal barrier during the initial stages of an infection. The aim of this study was to evaluate the potential of RFLP (restriction fragment length polymorphism) technique of gene inlA for monitoring the occurrence of potentially invasive strains of L. monocytogenes from different sources. The ability to invade the epithelial host cells of the intestinal barrier was detected in 52,3 % of the investigated strains. RFLP profiles (1and 4) associated with the production of truncated form of the internalin A and with the decreased invasive ability was detected only in serotype 1/2a and 1/2c. These RFLP profiles were find in strains isolated from food (43,6 %) and the external environment (66,7 %), but also in some human strains (42,1 %). Following of the inlA polymorphism by PCR-RFLP presents fast screening technique for evaluation of alteration in pathogenic potential L. monocytogenes. |