Publication details
Studium polymorfizmů genu RGH1a spojeného s rezistencí k padlí travnímu u planých ječmenů
| Title in English | Study of RGH1a gene polymorphisms associated with powdery mildew resistance in wild barley |
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| Authors | |
| Year of publication | 2011 |
| Type | Article in Proceedings |
| Conference | Genetická konference GSGM |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | mapping; SSR; RGH; powdery mildew; Blumeria graminis |
| Description | Three donors of powdery mildew resistance of wild barley (H. vulgare ssp spontaneum) were studied to identify resistance genes by genetic mapping and further molecular analysis. Genetic mapping in the segregating F2 population of the cross between the susceptible variety Tiffany and wild barley allowed to locate the resistance genes on chromosome 1H, near the locus Mla. This locus contains three gene families RGH1, RGH2 and RGH3 with eight race-specific genes (Wei et al. 2002). RGH1a locus was located 1cM from the resistance gene in the cross PI466200 x Tiffany, the phenotypic variance was 95%. In the cross Tiffany x PI466495 resistance gene was located 2cM from gene RGH1a and efficiency of the gene was 84%. The linkage between RGH1a and resistance gene has not been found in cross PI466510 x Tiffany. RGH1a gene was sequenced to find polymorphisms suitable for the development of DNA markers. Primers for sequencing were derived from RGH1a gene sequences (http://www.ncbi.nlm.nih.gov/). Identified polymorphisms (inserts, deletions and substitutions) allowed designing PCR-RFLP and the allele-specific markers. These markers permit both more precise localization of identified resistance genes, but also the design of association studies and monitoring of functional polymorphisms that cause a change or shift the reading frame. |
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