Publication details
Voltammetric determination of zinc(II) ions level in blood serum of prostate cancer patients revealing their relation with cancer progression
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| Year of publication | 2011 |
| Type | Conference abstract |
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| Citation | |
| Description | Prostate cancer is the second most frequently diagnosed cancer in developed countries. Prostatic tissue is a very specific in Zn2+ metabolism. Its cells accumulate up to tenfold higher quantity of these ions in compare with other tissues. The main topic of this study was sensitive determination of zinc(II) by differential pulse voltammetry (accumulation time 120 s et potential -1.2 V) in blood serum of patients with prostate cancer. Primarily, we optimized experimental conditions on manual measuring system and then we applied the conditions on fully automatic measuring system. Then, the simple and easy-to-use protocol for microwave-mineralization (80 W, 120 min) of low volume of blood samples (glass vials with 10 ul and HNO3) was suggested. The advantages and disadvantages of the procedure were discussed. The zinc(II) levels were low from tens to hundreds of nM. The differences between content of free and bounded zinc(II) were determined and correlated with progression of the disease and with common marker called prostatic specific antigen and with new marker called metallothionein. We found strong mutual correlation between them and zinc(II) ions. |