Publication details
Zinc, sarcosine and prostate? Is there any relation revealed by electrochemistry?
| Authors | |
|---|---|
| Year of publication | 2011 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Prostate cancer is the second most frequently diagnosed cancer in developed countries. Prostatic tissue is a very specific in Zn2+ metabolism. Sarcosine as non-toxic amino acid can be newly considered as a urine-marker of early stages of tumours and its connection with zinc(II) metabolism has not been studied yet. The aim of this study was to suggest fast and rapid method to determine sarcosine in human urine. The optimized experimental conditions were mobile phase from methanol and trifluoroacetic acid (80 mM) and working electrode potential 900 mV. Under these we were able to separate sarcosine from other molecules in urine of patients with prostate cancer. Limit of detection of this method was around 1 pg/ml and time of one analysis was 30 minutes. Further, we employed differential pulse voltammetry (accumulation time 120 s et potential -1.2 V) for determination of zinc(II) in blood serum of patients with prostate cancer. We correlated zinc(II) and sarcosine level with progression of the disease and with content of prostatic specific antigen and metallothionein. We found strong mutual correlation between them and zinc(II) ions. |