Zn-PROTEINS FROM STAPHYLOCOCCUS AUREUS BYMAGNETIC NANOPARTICLES FOLLOWED BY THEIR ELECTROPHORETIC

• Authors: JÍLKOVÁ Eva; KŘÍŽKOVÁ Soňa; HYNEK David; KREJČOVÁ Ludmila; SOCHOR Jiří; ADAM Vojtěch; HODEK Petr; TRNKOVÁ Libuše; STIBOROVÁ Marie; HUBÁLEK Jaromír; KIZEK René
• Year of publication: 2012
• Type: Article in Proceedings
• Conference: XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků.
• MU Faculty or unit: Faculty of Science
• Field: Physical chemistry and theoretical chemistry
• Keywords: zinc;proteins;staphylococcus aureus;nanoparticles
• Description: Automated immunoseparation of Zn-proteins from Staphylococcus aureus by magnetic nanoparticles followed by their electrophoretic is the aim of this paper. For isolation of cells S. aureus was used magnetic nanoparticles with specific antibodies. For electrophoretic detection of zinc binding proteins SDS-PAGE was used. For electrochemical determination measuring system Metrohm (Autosampler 813 Compact and measuring unit VA Computrace 797) was employed.