Publication details
Anaeroby v chronické ráně: banalita nebo skrytá hrozba?
| Title in English | Anaerobes in a chronic wound: banality or a hidden threat? |
|---|---|
| Authors | |
| Year of publication | 2012 |
| Type | Article in Periodical |
| Magazine / Source | Hojeni ran |
| MU Faculty or unit | |
| Citation | |
| Field | Surgery incl. transplantology |
| Keywords | anaerobes chronic wound |
| Description | Aim To establish the prevalence of particular anaerobic bacteria in patients with chronic wound. Material and methodology All patients, hospitalized with a chronic wound in the Clinic of burns and reconstructive surgery of Faculty Hospital of Brno in the period from January 1st, 2008 till December 31st, 2010, were enrolled in our data set. In these patients, the material was collected by regular and targeted collection from skin defects. A swab made with a sterile cotton wool swab subsequently dipped into transport medium, which enables a survival of anaerobic bacteria, plays an insubstitutable role in a diagnostic procedure of anaerobic etiological agents of chronic defect infections. This method is preferred to an imprinting. Following the transport to microbiologic laboratory, the material is inoculated in culture media including VL agar for a growth of anaerobic bacteria. The cultivation of anaerobic germs demands an anaerobic environment and a time period of 48 hours. The subsequent identification of anaerobes is performed by a set of diagnostic discs and diagnostic kits Remel and API bioMérieux. Results In total, 95 patients (32 women) with positive isolation of anaerobic bacteria in a chronic defect, who were hospitalized in the monitored period, were enrolled in our group. 75 patients in the group (78.95%) were hospitalized with an initial diagnosis of the pressure ulcer, 13 patients (13.68%) with the crural ulcer and 7 patients (7.36%) with a diabetic defect of lower extremity (the diabetic foot). M:F ratio 2.97:1. There were a total of 198 anaerobic bacteria tribes isolated in these patients. The most common anaerobic bacteria isolated from a chronic wound is Bacteroides fragilis 115 tribes in total (58.08%), followed by Peptococcus sp. 20 tribes (10.10%), Peptostreptococcus sp. 19 tribes (9.60%), and Prevotela melaninogenica 14 tribes (7.07%). Conclusion In our study we established the prevalence of basic anaerobic bacteria in patients with chronic wound. We also proved the fact that the highest incidence of anaerobes is in patients with pressure ulcers. |