Publication details
Indikace dopadů stresu na vitalitu in-vitro kultivovaných rostlin pomocí pokročilých metod fluorescence chlorofylu
| Title in English | Indication of stress effects on vitality of in vitro cultivated plants by advanced chlorophyll fluorescence methods |
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| Authors | |
| Year of publication | 2011 |
| Type | Article in Proceedings |
| Conference | ROSTLINNÉ BIOTECHNOLOGIE - II. Současné trendy ve výuce a výzkumu |
| MU Faculty or unit | |
| Citation | |
| Web | web stranka sborniku |
| Field | Botany |
| Keywords | in vitro; micropropagation; chlorophyll fluorescence; photosynthetic parameters; ex vitro transfer |
| Description | Chlorophyll fluorescence technique represents an important tool for estimation of plant photosynthetic performance and evaluation of effects of particular stress factors on plant vitality. It is widely-exploited method in photosynthetic studies of higher plants and other photosynthetizing organisms such as e.g. cyanobacteria, algae, lichens and mosses. Recently, there are numerous application of chlorophyll fluorescence ranging from purely photosynthetic to ecophysiological and plant stress studies. Among them, short- and long- term photosynthetic measurements in the field, pre- and post-harvest control of fruits, growth and vitality control in algal biotechnologies are of great importance. In plant tissue cultures and in vitro cultivated plants, the method has met less frequent exploitation than in naturally-grown plants. Since the 90-ies of the last century, however, an increasing number of fluorometric studies in in vitro plants has been published. Recently, variety of methods based on chlorophyll fluorescence technique is used in in vitro studies. This paper brings a theoretical background, overview of recently used fluorometric methods, and commented examples of their typical applications. The emphasis is given to the most recent and advanced techniques, the use of which is expected to expand in the nearest future. |