Screening a cDNA Library for Protein-Protein Interactions Directly in Planta

• Authors: LEE Lan-Ying; WU Fu-Hui; HSU Chen-Tran; SHEN Shu-Chen; YEH Hsuan-Yu; LIAO De-Chih; FANG Mei-Jane; LIU Nien-Tze; YEN Yu-Chen; DOKLÁDAL Ladislav; SÝKOROVÁ Eva; GELVIN Stanton B.; LIN Choun-Sea
• Year of publication: 2012
• Type: Article in Periodical
• Magazine / Source: Plant Cell
• MU Faculty or unit: Central European Institute of Technology
• web: http://www.plantcell.org/content/24/5/1746.abstract
• Doi: http://dx.doi.org/10.1105/tpc.112.097998
• Field: Genetics and molecular biology
• Keywords: BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STRAND TELOMERIC DNA; BINDING-PROTEIN; NUCLEAR IMPORT; IN-PLANTA; T-DNA; VIRD2 PROTEIN; CELLS; TRANSFORMATION; VISUALIZATION
• Description: Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. T-DNA insertions in the genes represented by some cDNAs revealed five novel Arabidopsis proteins important for Agrobacterium-mediated plant transformation. We also used this cDNA library to confirm VirE2-interacting proteins in orchid (Phalaenopsis amabilis) flowers. Thus, this technology can be applied to several plant species.

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