Publication details
Eliminační voltametrie krátkých oligodeoxynukleotidů
| Title in English | Elimination voltammetry of short oligodeoxynucleotides |
|---|---|
| Authors | |
| Year of publication | 2012 |
| Type | Article in Proceedings |
| Conference | Sborník přednášek a posterů, program |
| MU Faculty or unit | |
| Citation | |
| Field | Physical chemistry and theoretical chemistry |
| Keywords | DNA;elimination voltammetry;oligodeoxynucleotides;EVLS;heptamer |
| Description | Electrochemical research of DNA and oligodeoxynucleotides (ODN) is a recently received considerable attention, both because of the possibility of a deeper understanding of their structure and function of the charged phase interfaces, both because of the possibility of application of electroanalytical methods in biomedicine for diagnostic purposes. The advantage of electroanalytical approach is simpler and less expensive instrumentation, less time-consuming, the ability of automation, miniaturization and parallel analysis of multiple samples (sensor array); disadvantage in some cases, the sensitivity and selectivity, which can increase the voltammetric methods using elimination voltammetry (EVLS - Elimination Voltammetry with Linear Scan). EVLS is the transformation procedure using different sub voltammetric currents depending on the scan rate of polarization. The result of the elimination of undesirable and maintain the desired current components, the elimination function expressed as a linear combination of the total voltammetric currents measured at different speeds polarization. EVLS is able to significantly increase the current sensitivity, separation of overlapping voltammetric signals, reveal minority processes hidden in the majority process, expand potential window and help solve the mechanism of electrode processes. It is also capable of sensitively and rapidly detect electroactive particles adsorbed state (peak-contrepeak). EVLS been successfully used in the analysis of short homo-and hetero-ODN. Besides nonamers, which they found sequentially dependent reduction depending cytosine and adenine signals at mercury electrode were studied heptamers, including a very stable DNA fragment d(GCGAAGC). It has been shown that EVLS in conjunction with adsorptive stripping technique is a suitable tool electroanalytical ODN study of structural changes on the surface of mercury and carbon electrodes. |