Publication details
Visualization, tracking and quantification of bone-marrow mesenchymal cells homing in rabbit heart(nanoparticles labeling and imaging by magnetic resonance and SPECT)
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Conference abstract |
| Citation | |
| Description | Background and objectives: : Monitoring of stem cell homing plays a crucial role in preclinical evaluation of evolving stem cells therapies. Stem cells are wandering of desired organs and tissues very often. In case of heart is essential to visualize the right position of the delivered cells and ideal to quantify (recognize) the amount of remaining cells in the infarcted area. Most common were histological methods of recognizing remaining cells. Noninvasive methods would be more efficient and less time consuming. For tracking is very important to label the cells with particle which does not effect viability and would be specific enough to mark even a small number of cells. Bone-marrow mesenchymal cells (BMMCs) labeling methods were already described for canine, pig and rat models. Evaluation and comparison of labeling methods for rabbit BMMC is still lacking. Nuclear magnetic resonance is common cardiovascular diagnostic method with ideal recognition of heart geometry and motion. Radioactive indium can be tracked for four weeks and amount of radioactivity in the tissue is measurable. Combination of these two methods seems to be ideal. Methods: Rabbit BMMCs were isolated and cultured for 1-3 weeks. (A) Labeling was based on incubation of cells with complex 111 Indium-tropolone for 5-15 min. Labeling efficiency was determined. Surviving of BMMCs during 1 week was monitored. Different amounts of labeled cells were placed in phantom of rabbit chest and underwent basic gamma kamera imaging. (B) Two types of iron oxide particles (Resovist or supermagnetic maghemite) were added to the BMMCs culture up to final concentration 100ug/ml. After several days particles were washed out and labeled BMMCs were placed in phantom of rabbit chest. Results NMR imaging was evaluated. BMMCs displaying about 3 Bq/cell. Viability was not significantly decreased by this procedure. BMMCs numbers as low as 50 x 10^3 could be easily localized and imaged using gamma camera.Resovist and deadherence process resulted in surviving of 80% of cells. Cluster as small as 50 x 10^3 cells could be detected by NMR imaging. Conclusion: Resovist labeling of rabbit BMMCs were proved working and optimized. These methods can be used as base for in vivo studies of tracking of cells delivered into the infarcted rabbit heart. Washout of radioactive/contrast substances does not affect our methods and viability of the cells is not impaired. |