Informace o projektu
Aberrant Splicing of CFTR Exon 9
(ASCE)
- Kód projektu
- 630758
- Období řešení
- 3/2014 - 2/2018
- Investor / Programový rámec / typ projektu
-
Evropská unie
- 7. rámcový program EU
- Lidé
- Fakulta / Pracoviště MU
- Středoevropský technologický institut
Alternative splicing of pre-mRNAs is tightly regulated and any imbalance can change the outcome of gene expression which often leads to disease. Despite the importance of alternative splicing regulation our knowledge at the molecular level is still in its infancy hampering development of effective therapies. We plan to study the regulatory RNA-protein and proteinprotein interaction networks leading to skipping of CFTR exon 9 associated with severe forms of cystic fibrosis. Structure
determination of regulatory complexes by NMR spectroscopy in combination with biochemical and functional in vivo studies will be used to deepen our molecular understanding of the regulatory networks governing the proper assembly of mRNAs.
With this, we want to reveal the molecular basis of aberrant CFTR exon 9 splicing and lay a rational, structure-functionbased
foundation for novel approaches to cure diseases.
Publikace
Počet publikací: 3
2020
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Cancer-Associated Substitutions in RNA Recognition Motifs of PUF60 and U2AF65 Reveal Residues Required for Correct Folding and 3 ' Splice-Site Selection
Cancers, rok: 2020, ročník: 12, vydání: 7, DOI
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Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition
Nucleic acids research, rok: 2020, ročník: 48, vydání: 4, DOI
2016
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Efficient large-scale preparation and purification of short single-stranded RNA oligonucleotides
Biotechniques, rok: 2016, ročník: 60, vydání: 2, DOI