Informace o projektu
Cell interactions in tumour microenvironment as a new tool for therapy response prediction in head and neck cancer
- Kód projektu
- NV18-08-00229
- Období řešení
- 5/2018 - 12/2021
- Investor / Programový rámec / typ projektu
-
Ministerstvo zdravotnictví ČR
- Program na podporu zdravotnického aplikovaného výzkumu a vývoje na léta 2015 - 2022
- Fakulta / Pracoviště MU
- Lékařská fakulta
- Spolupracující organizace
-
Fakultní nemocnice v Motole
- Odpovědná osoba prof. MUDr. Jan Betka, DrSc., FCMA
Tumour microenvironment (TME) can activate protecting signalling pathways in tumour cells, especially when TME cells are damaged by chemotherapy. In this project, we will focus on cisplatin resistance conferred to cancer cells by TME. FaDu cells will be co-cultured with primary cell lines derived from tumour tissue of individual head and neck cancer (HNSCC) patients. Effect of co-culture on resistance, invasiveness, and migratory capacity of cancer cells will be assessed. Based on those, we will propose a protocol for detection of tumour-supporting stroma in individual HNSCC patients, which is an important step in personalized medicine of HNSCC. Group of cultures well-supporting and non-supporting chemoresistance will be then analysed for differences in cell stiffness (assessed by atomic force microscopy) and for changes in gene expression (microarray technology) and amino acid profiles (ion-exchange liquid chromatograph). The main aim is to select reliable markers of tumour-supporting stroma detectable in biological samples of HNSCC patients.
Publikace
Počet publikací: 8
2022
-
mRNA Subtype of Cancer-Associated Fibroblasts Significantly Affects Key Characteristics of Head and Neck Cancer Cells
Cancers, rok: 2022, ročník: 14, vydání: 9, DOI
2021
-
Novel Mitochondria-targeted Drugs for Cancer Therapy
Mini-reviews in medicinal chemistry, rok: 2021, ročník: 21, vydání: 7, DOI
-
Sensitivity to Cisplatin in Head and Neck Cancer Cells Is Significantly Affected by Patient-Derived Cancer-Associated Fibroblasts
International Journal of Molecular Sciences, rok: 2021, ročník: 22, vydání: 4, DOI
-
The impact of mRNA subtype of cancer-associated fibroblasts on metabolic symbiosis in head and neck cancer
Rok: 2021, druh: Konferenční abstrakty
2020
-
Caveolin-1 in oncogenic metabolic symbiosis
International journal of cancer, rok: 2020, ročník: 147, vydání: 7, DOI
2019
-
Effect of tumor microenvironment on pathogenesis of the head and neck squamous cell carcinoma: a systematic review
Molecular Cancer, rok: 2019, ročník: 18, vydání: 63, DOI
-
Prognostic Significance of Serum Free Amino Acids in Head and Neck Cancers
Cells, rok: 2019, ročník: 8, vydání: 5, DOI
-
Unexpected therapeutic effects of cisplatin
Metallomics, rok: 2019, ročník: 11, vydání: 7, DOI