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Informace o projektu
Finding unknown endocrine disrupting compounds through target pull-down assay filtration, effect direct analysis and ultra-high resolution mass spectrometry for a comprehensive efficient workflow
(PullEd-MS)
- Kód projektu
- 839243
- Období řešení
- 11/2019 - 10/2022
- Investor / Programový rámec / typ projektu
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Evropská unie
- Horizon 2020
- MSCA Marie Skłodowska-Curie Actions (Excellent Science)
- Fakulta / Pracoviště MU
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Přírodovědecká fakulta
- prof. RNDr. Jana Klánová, Ph.D.
- Garry Paul Codling, Dr.
There are thousands of anthropogenic compounds in general use across the globe and every year more chemicals are created. For every compound, that finds its way into the environment multiple degradation compounds can result due to environmental processes. These changes alter the toxicological properties and chemical mobility creating potentially unpredicted effects. Environmental monitoring using effect direct analysis (EDA) to identify toxicological endpoints combined with mass spectrometry (MS) to detect the most abundant active compounds is an established methodology. The latest MS instruments the ultra-high resolution MS (UHRMS) have allowed full scan acquisition to become the cutting edge in the detection and identification of unknown compounds. However, knowing which EDA to apply is still a challenge and when UHRMS scans detect thousands of compounds identifying which compounds caused the toxicological response is a challenge. This MSC proposal aims to develop the use of pull-down assays linked to specific nuclear receptors (NR) such as estrogen receptor α as a filter to bind only those chemicals with an affinity for that specific protein. By then applying only the target specific EDA and fractionation to identify the most bioactive fraction before UHRMS a more focused workflow for compound detection based on effect can be developed. This workflow will be tested using samples of varying complexity including wastewater, sediment, and human biological samples. These workflows will then be tested using water collected from the Global Water Network to identify novel compounds of toxicological effect in the environment and using human urine from pregnant mothers collected from the CELSPAC-TNG cohort for identification of human exposure. In developing this comprehensive methodology, the sample will have specific toxicological effects identified as well as chemical investigation providing a thorough NR specific determination of both known and unknown compounds.
Publikace
Počet publikací: 4
2022
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Application of 96-well plate SPE method for analysis of persistent organic pollutants in low volume blood serum samples
Chemosphere, rok: 2022, ročník: 287, vydání: January 2022, DOI
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LC-HRMS based method for suspect/non-targeted screening for biomarkers of chemical exposure in human urine
Chemosphere, rok: 2022, ročník: 300, vydání: August 2022, DOI
2021
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Molecular mechanisms of zooplanktonic toxicity in the okadaic acid- producing dinoflagellate Prorocentrum lima
Environmental Pollution, rok: 2021, ročník: 279, vydání: June 2021, DOI
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Remodeling of Arctic char (Salvelinus alpinus) lipidome under a stimulated scenario of Arctic warming
Global Change Biology, rok: 2021, ročník: 27, vydání: 14, DOI