Informace o projektu
Elucidating the role of the RNA editing enzyme ADAR1 in novel biological pathways and determine its posttranslational regulation.
- Kód projektu
- GA20-11101S
- Období řešení
- 1/2020 - 12/2022
- Investor / Programový rámec / typ projektu
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Grantová agentura ČR
- Standardní projekty
- Fakulta / Pracoviště MU
- Středoevropský technologický institut
ADARs converts adenosine to inosine in double-stranded RNA and this is one of the most abundant modifications found in RNA. Previously, my group has demonstrated the pivotal role of ADAR1 in innate immunity. However there is also an editing independent function of ADAR1 that is important but currently unknown. We propose to uncover this editing independent function by investigating proteins that interact with ADAR1. We have preliminary data on the interaction of ADAR1 with differential protein complexes when cells are treated with α-interferon. We will analyse this data, validate protein interactions and map which biological pathways ADAR1 has a direct influence on and whether it depends on the enzymatic activity.
It has been predicted that ADAR1 is highly modified posttranslationally and this may account for variation in its activity in different cell types. The second aim is to elucidate if these posttranslational modifications regulate ADAR1, in particular those modifications of ADAR1 that occur after type 1 interferon induction.
Cíle udržitelného rozvoje
Masarykova univerzita se hlásí k cílům udržitelného rozvoje OSN, jejichž záměrem je do roku 2030 zlepšit podmínky a kvalitu života na naší planetě.
Publikace
Počet publikací: 7
2024
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Amyloid precursor protein induces reactive astrogliosis
Acta Physiologica, rok: 2024, ročník: 240, vydání: 6, DOI
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An ADAR1 dsRBD3-PKR kinase domain interaction on dsRNA inhibits PKR activation
Cell Reports, rok: 2024, ročník: 43, vydání: 8, DOI
2022
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ADAR2 enzymes: efficient site-specific RNA editors with gene therapy aspirations
RNA, rok: 2022, ročník: 28, vydání: 10, DOI
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The mechanical regulation of RNA binding protein hnRNPC in the failing heart
Science Translational Medicine, rok: 2022, ročník: 14, vydání: 672, DOI
2021
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ADAR RNA Modifications, the Epitranscriptome and Innate Immunity
Trends in Biochemical Sciences, rok: 2021, ročník: 46, vydání: 9, DOI
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Biallelic variants in ADARB1, encoding a dsRNA-specific adenosine deaminase, cause a severe developmental and epileptic encephalopathy
Journal of Medical Genetics, rok: 2021, ročník: 58, vydání: 7, DOI
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The effects of RNA editing in cancer tissue at different stages in carcinogenesis
RNA Biology, rok: 2021, ročník: 18, vydání: 11, DOI