Informace o projektu
Interactions defining physiological functions of Microtubule Associated Protein 2c at atomic resolution
- Kód projektu
- GA20-12669S
- Období řešení
- 1/2020 - 12/2022
- Investor / Programový rámec / typ projektu
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Grantová agentura ČR
- Standardní projekty
- Fakulta / Pracoviště MU
- Středoevropský technologický institut
Microtubule associated protein 2c (MAP2c) is a key factor regulating microtubule dynamics in
developing brain neurons, and an example of an intrinsically disordered proteins with an
important physiological function and detectable structure-function relationship. We propose to
study interactions of MAP2c defining and controlling its biological function. We focus on two
regions in the N-terminal half of MAP2c sequence that bind molecules not interacting with the
homologous protein Tau and contain structural motifs unique for MAP2c and not found in Tau.
We will study interactions with steroids, regulatory subunit of kinase PKA, plectin, and evaluate
effects of phosphorylation and interactions with regulatory 14-3-3 proteins. We also propose to
study impact of the interactions on microtubule binding and address biological relevance of the
in vitro results by recording NMR spectra at near-to-native conditions (in cells and/or cell
lysates) and performing cryo-electron tomography on monolayered neurons to observed
interactions of cytoskeleton components regulated by MAP2c binding in situ.
Cíle udržitelného rozvoje
Masarykova univerzita se hlásí k cílům udržitelného rozvoje OSN, jejichž záměrem je do roku 2030 zlepšit podmínky a kvalitu života na naší planetě.
Publikace
Počet publikací: 3
2024
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Structural basis of binding the unique N-terminal domain of microtubule-associated protein 2c to proteins regulating kinases of signaling pathways
International Journal of Biological Chemistry, rok: 2024, ročník: 300, vydání: 8, DOI
2022
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Interactions of adaptor protein Grb2 with microtubule associated protein 2c
Rok: 2022, druh: Další prezentace na konferencích
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Specific phosphorylation of microtubule-associated protein 2c by extracellular signal–regulated kinase reduces interactions at its Pro-rich regions
JOURNAL OF BIOLOGICAL CHEMISTRY, rok: 2022, ročník: 298, vydání: 10, DOI