HILIC SEPARATION OF SARCOSINE HYPHENATED TO COULOMETRIC DETECTION

• Autoři: CERNEI Natalia Vladimirovna; KOMINÍKOVÁ Markéta; ZÍTKA Ondřej; GUMULEC Jaromír; MASAŘÍK Michal; ADAM Vojtěch; HUBÁLEK Jaromír; TRNKOVÁ Libuše; STIBOROVÁ Marie; ECKSCHLAGER Tomáš; KIZEK René
• Rok publikování: 2012
• Druh: Konferenční abstrakty
• Popis: Recently, sarcosine has been identified as a potential prostate cancer marker. To provide early determination of this compound, number of methods is still developed. Sarcosine is relatively small molecule, which is polar enough to enable separation by chromatography with reverse phase. For this reason we optimized method for separation by Hydrophilic interaction chromatography (HILIC). The aim of this work was to suggest the method for HILIC separation and determination of sarcosine with electrochemical detection. This aim was reached as shown by the limit of detection (350 nM), which was determined from calibration curve. This method is suitable for determination of sarcosine in real samples such as urine.