Informace o publikaci
MONITORING THE VIABILITY OF CELL LINE MTF-KO DEPENDING ON THE CONCENTRATION OF Zn2+ USING XCELLIGENCE SYSTEM
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| Rok publikování | 2012 |
| Druh | Konferenční abstrakty |
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| Popis | Metallothioneins (MTs) are low-molecular mass cysteine-rich proteins. Due to thiol bounds in its structure is metallothionein capable to bind metals and thus play a role in metal transportation, cell detoxification and oxidative stress protection. Metallothionein is closely related to zinc metabolism, as zinc is essential compound of mammalian living cells. MT transcription is mediated and controlled thru MTF-1 factor which is activated by metal caused oxidative stress. It is obvious that MTF-1 plays role not only in detoxification and oxidative stress protection, but also in ontogenetic processes. The cell line used in our study was obtained by knock-out of metal regulatory transcription factor 1 (MTF-1) in mouse embryonic fibroblast cell culture MEF-1. We focused on viability monitoring of MTF-KO cell culture viability after zinc ion treatments. This cell line has knock-out metal regulatory transcription factor 1 (MTF-1). Compared to wild type form of mouse embryonic fibroblasts, the growth of MTF-KO cell line is inhibited at lower zinc concentrations. |
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