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Staphylococcus petrasii sp. nov. including S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov., isolated from human clinical specimens and human ear infections
Název česky | Staphylococcus petrasii sp. nov. including S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov., izolovaný z klinických vzorků humánního původu a lidských ušních infekcí |
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Autoři | |
Rok publikování | 2013 |
Druh | Článek v odborném periodiku |
Časopis / Zdroj | Systematic and Applied Microbiology |
Fakulta / Pracoviště MU | |
Citace | |
www | http://www.sciencedirect.com/science/article/pii/S0723202012001579 |
Doi | http://dx.doi.org/10.1016/j.syapm.2012.11.004 |
Obor | Mikrobiologie, virologie |
Klíčová slova | Coagulase-negative Staphylococci; Staphylococcus petrasii; Otitis externa; Molecular phylogeny; Polyphasic taxonomy |
Přiložené soubory | |
Popis | Thirteen coagulase-negative, oxidase-negative, and novobiocin-susceptible staphylococci were isolated from human clinical specimens. The isolates were differentiated from known staphylococcal species on the basis of 16S rRNA, hsp60, rpoB, dnaJ, tuf, and gap gene sequencing, automated ribotyping, (GTG)5-PCR fingerprinting, and MALDI-TOF MS analysis. Phylogenetic analysis based on the 16S rRNA gene sequence indicated phylogenetic relatedness of the analyzed strains to Staphylococcus haemolyticus, Staphylococcus hominis, Staphylococcus devriesei, and Staphylococcus lugdunensis. DNA–DNA hybridization experiments between representative strains CCM 8418T, CCM 8421T, and the closest phylogenetic neighbors confirmed that the isolates represent novel Staphylococcus species, for which the name Staphylococcus petrasii sp. nov. is proposed. Genotypic and phenotypic analyses unambiguously split the strains into two closely related subclusters. Based on the results, two novel subspecies S. petrasii subsp. petrasii subsp. nov. and S. petrasii subsp. croceilyticus subsp. nov. are proposed, with type strains CCM 8418T (=CCUG 62727T) and CCM 8421T (=CCUG 62728T), respectively. |
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