The PI3K/AKT Signaling Pathway Controls the Quiescence of the Low-Rhodamine123-Retention Cell Compartment Enriched for Melanoma Stem Cell Activity

Informace o publikaci

Autoři	TOUIL Yasmine ZULIANI Thomas WOLOWCZUK Isabelle KURANDA Klaudia MEDALOVÁ Jiřina ANDRIEUX Joris LE ROY Helene MORTIER Laurent VANDOMME Jerome JOUY Nathalie MASSELOT Bernadette SEGARD Pascaline QUESNEL Bruno FORMSTECHER Pierre POLAKOWSKA Renata
Rok publikování	2013
Druh	Článek v odborném periodiku
Časopis / Zdroj	Stem Cells
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	URL
Doi	http://dx.doi.org/10.1002/stem.1333
Obor	Fyziologie
Klíčová slova	Melanoma stem cells; quiescence;Rhodamine123 exclusion ; LY294002; PI3K/AKT pathway ; phenotype switching
Popis	Melanoma is one of the most aggressive and extremely resistant to conventional therapies neoplasms. Recently, cellular resistance was linked to the cancer stem cell phenotype, still controversial and not well defined. In this study, we utilized a Rhodamine 123 (Rh123) exclusion assay to functionally identify stem-like cells in metastatic human melanomas and melanoma cell lines. We demonstrate that a small subset of Rh123-low-retention (Rh123low) cells is enriched for stem cell-like activities, including the ability to self-renew and produce non-stem Rh123high progeny and to form melanospheres, recapitulating the phenotypic profile of the parental tumor.

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