Informace o publikaci
Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Acta crystallographica. Section F |
| Fakulta / Pracoviště MU | |
| Citace | |
| Doi | http://dx.doi.org/10.1107/S1744309113002522 |
| Obor | Biochemie |
| Klíčová slova | Mannosidase; Aspergillus niger; Expression; Crystallization |
| Popis | beta-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various oligomeric saccharide structures. beta-Mannosidase from Asperigillus niger was expressed in Pichia pastoris and purified to clear homogeneity. beta-mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 A resolution. The crystal belonged to space group P1. Monomeric structure was observed in the asymmetric unit. |
| Související projekty: |