Informace o publikaci
Paramagnetic Particles Isolation of Influenza Oligonucleotide Labelled with CdS QDs
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Chromatographia |
| Fakulta / Pracoviště MU | |
| Citace | |
| Doi | http://dx.doi.org/10.1007/s10337-012-2327-0 |
| Obor | Fyzikální chemie a teoretická chemie |
| Klíčová slova | Biosensors; Voltammetry; Automated separation; Nanoparticles; Quantum dots; Hybridization; Virus |
| Popis | In this study, we describe hybridization design probes consisting of paramagnetic particles and quantum dots (QDs) with targeted DNA, and their application for detection of avian influenza virus (H5N1). Optical properties of QDs were beneficial, but the main attention was paid to the electroactivity of metal part of QDs and ODNs themselves. Differential pulse voltammetry was used for detection of cadmium(II) ions and square wave voltammetry for detection of cytosine-adenine peak in ODN-SH-Cd complex. It clearly follows from the obtained results that the optimized conditions were temperature of hybridization 25 A degrees C, time of hybridization 35 min, and concentration of ODN-SH-Cd complex 20 mu g mL(-1). The detection limit (3 signal/noise) was estimated as 15 ng mL(-1) of ODN-SH-Cd. |