Informace o publikaci
Determination of asymmetric and symmetric dimethylarginines in human plasma using high-performance liquid chromatography with electrochemical detection
| Název česky | Stanovení asymetrického a symetrického dimethylargininu v lidské plazmě pomocí HPLC s elektrochemickou detekcí |
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| Autoři | |
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Separation Science |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://onlinelibrary.wiley.com/doi/10.1002/jssc.201300813/abstract |
| Doi | http://dx.doi.org/10.1002/jssc.201300813 |
| Obor | Biochemie |
| Klíčová slova | L Arginine; Asymmetric dimethylarginine; Electrochemical detection; HPLC; Symmetric dimethylarginine |
| Přiložené soubory | |
| Popis | A new HPLC method was developed and validated for the determination of asymmetric and symmetric dimethylarginines and L arginine in human plasma. After a SPE and evaporation of the eluate, the samples were derivatized with an o-phthaldialdehyde reagent containing 3 mercaptopropionic acid. The derivatives formed were analysed by an isocratic RP HPLC and electrochemical detection at +320 mV. |
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