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IMPROVING THE REPEATABILITY OF SAMPLING PROCEDURES FOR EXHALED BREATH CONDENSATE ANALYSIS

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GREGUŠ Michal KUBÁŇ Petr FORET František

Rok publikování 2013
Druh Článek ve sborníku
Konference Chemické listy
Fakulta / Pracoviště MU

Středoevropský technologický institut

Citace
www Chemické listy
Obor Analytická chemie, separace
Klíčová slova capillary electrophoresis; contactless conductivity detection; exhaled breath condensate; sampling device; standardization
Popis A miniature sampler for exhaled breath condensate (EBC) collection was constructed and the repepatability of the collection procedure was studied. The samples were analyzed by CE-C4D home-made instrument. To improve the repeatability of the EBC collection, first the collection tubes, straws and vials require cleanup to remove unwanted interferents. Second, the breathing pattern should be kept uniform. We demonstrate that by adopting these measures, repeatability can be improved from 21.4– 186.5 % RSD (non-standardized sampling) to 3.6–80.3 % RSD (standardized sampling) and is comparable than with the commercial device (6.6–75.6 % RSD).
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