Informace o publikaci
Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling
| Autoři | |
|---|---|
| Rok publikování | 2013 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Chemical communications |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://www.rsc.org/chemcomm |
| Doi | http://dx.doi.org/10.1039/C3CC41438H |
| Obor | Biochemie |
| Klíčová slova | DNA polymerase; DNA labelling; oligonucleotides |
| Popis | Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template. |
| Související projekty: |