Informace o publikaci

MDM2 polymorphism associated with the development of cervical lesions in women infected with Human papillomavirus and using of oral contraceptives

Autoři	AMARAL Carolina M.M. CETKOVSKÁ Kateřina GURGEL Ana P.A.D. CARDOSO MV CHAGAS Bárbara S. PAIVA Sérgio S.L. LIMA Rita de Cássia Pereira d SILVA-NETO Jacinto C. SILVA Luiz A.F. MUNIZ Maria T.C. BALBINO Valdir Q. FREITAS Antonio C.
Rok publikování	2014
Druh	Článek v odborném periodiku
Časopis / Zdroj	Infectious Agents and Cancer
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	http://www.infectagentscancer.com/content/9/1/24
Doi	http://dx.doi.org/10.1186/1750-9378-9-24
Obor	Onkologie a hematologie
Klíčová slova	MDM2; HPV; Oral contraceptives; Cervical cancer
Popis	Background: The MDM2 gene is the major negative regulator of p53, a tumor suppressor protein. Single nucleotide polymorphism in promoter region of MDM2 gene leads to increased expression resulting in higher levels of MDM2 protein. This event increases the attenuation of the p53 pathway. Polymorphisms in this gene can interfere in the regulation of cellular proliferation. We evaluated whether MDM2 SNP309 (rs2278744) associated or not with the use of oral contraceptive can heighten susceptibility to development of cervical lesions in women HPV infected. Methods: MDM2 SNP309 (rs2278744) was genotyped in a total of 287 patients using the PCR-RFLP technique. The results were analyzed by UNPHASED v.3.121 and SNPStats programs. Results: The three groups (SIL, LSIL and HSIL) showed no significant differences in either genotype or allelic frequencies for MDM2 polymorphisms, except when HSIL was compared with LSIL (p = 0.037; OR = 1.81). Furthermore, in the analysis of contraceptives, a significant association was found between the use of contraceptives and the MDM2 variant in the development of high-grade cervical lesions for the TG genotype (p = 0.019; OR = 2.21) when HSIL was compared with control. When HSIL was compared with LSIL (p = 0.006; OR = 2.27). Conclusion: The results of this study suggest that MDM2 SNP309 might be a good marker for assessing the progression of LSIL to HSIL. In addition, they also show that oral contraceptives alone, did not have any effect on the progression or development of cervical lesions. However, they may act synergistically with MDM2 SNP309 (rs2278744) and HPV infection in the development of cervical lesions.