Informace o publikaci

Are In Vitro Methods for the Detection of Endocrine Potentials in the Aquatic Environment Predictive for In Vivo Effects? Outcomes of the Projects SchussenAktiv and SchussenAktiv plus in the Lake Constance Area, Germany

Autoři	HENNEBERG A. BENDER K. BLÁHA Luděk GIEBNER S. KUCH B. KOHLER H.R. MAIER D. OEHLMANN J. RICHTER D. SCHEURER M. SCHULTE-OEHLMANN U. SIERATOWICZ A. ZIEBART S. TRIEBSKORN R.
Rok publikování	2014
Druh	Článek v odborném periodiku
Časopis / Zdroj	Plos one
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0098307
Doi	http://dx.doi.org/10.1371/journal.pone.0098307
Obor	Znečištění a kontrola vody
Klíčová slova	TREATMENT-PLANT EFFLUENTS; MUNICIPAL SEWAGE PLANT; UNITED-KINGDOM RIVERS; TROUT SALMO-TRUTTA; E-SCREEN ASSAY; POTAMOPYRGUS-ANTIPODARUM; ESTROGENIC ACTIVITY; WASTE-WATER; DISRUPTING CHEMICALS; BISPHENOL-A
Popis	Many studies about endocrine pollution in the aquatic environment reveal changes in the reproduction system of biota. We analysed endocrine activities in two rivers in Southern Germany using three approaches: (1) chemical analyses, (2) in vitro bioassays, and (3) in vivo investigations in fish and snails. Chemical analyses were based on gas chromatography coupled with mass spectrometry. For in vitro analyses of endocrine potentials in water, sediment, and waste water samples, we used the E-screen assay (human breast cancer cells MCF-7) and reporter gene assays (human cell line HeLa-9903 and MDA-kb2). In addition, we performed reproduction tests with the freshwater mudsnail Potamopyrgus antipodarum to analyse water and sediment samples. We exposed juvenile brown trout (Salmo trutta f. fario) to water downstream of a wastewater outfall (Schussen River) or to water from a reference site (Argen River) to investigate the vitellogenin production. Furthermore, two feral fish species, chub (Leuciscus cephalus) and spirlin (Alburnoides bipunctatus), were caught in both rivers to determine their gonadal maturity and the gonadosomatic index. Chemical analyses provided only little information about endocrine active substances, whereas the in vitro assays revealed endocrine potentials in most of the samples. In addition to endocrine potentials, we also observed toxic potentials (E-screen/reproduction test) in waste water samples, which could interfere with and camouflage endocrine effects. The results of our in vivo tests were mostly in line with the results of the in vitro assays and revealed a consistent reproduction-disrupting (reproduction tests) and an occasional endocrine action (vitellogenin levels) in both investigated rivers, with more pronounced effects for the Schussen river (e. g. a lower gonadosomatic index). We were able to show that biological in vitro assays for endocrine potentials in natural stream water reasonably reflect reproduction and endocrine disruption observed in snails and field-exposed fish, respectively.