Informace o publikaci
Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite
| Autoři | |
|---|---|
| Rok publikování | 2015 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | CHEMICAL SCIENCE |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://pubs.rsc.org/en/content/articlelanding/2015/sc/c4sc03983a#!divAbstract |
| Doi | http://dx.doi.org/10.1039/c4sc03983a |
| Obor | Analytická chemie, separace |
| Klíčová slova | APPENDED BIPYRIDINIUM SALTS; BORONIC ACID; HYDROGEN-PEROXIDE; NITRIC-OXIDE; FLUORESCENT-PROBES; SENSING SYSTEM; GLUCOSE; CHEMOSENSOR; REPORTER; VIOLOGEN |
| Popis | Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS–NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B–N interaction. Our simple system produces a visible colorimetric change and on–off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reactionbased Indicator displacement Assay). |
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